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M.R.S BROTH (DE MAN, ROGOSA, SHARPE) OXOID
MRS BROTH (DE MAN, ROGOSA, SHARPE)
CM0359
Add 52g to 1 litre of distilled water at approximately 60°C. Mix until completely dissolved. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.
MRS BROTH (DE MAN, ROGOSA, SHARPE)
Code: CM0359
A non-selective medium for profuse growth of `lactic acid bacteria’.
| Typical Formula* |
gm/litre |
| Peptone |
10.0 |
| `Lab-Lemco’ powder |
8.0 |
| Yeast extract |
4.0 |
| Glucose |
20.0 |
| Sorbitan mono-oleate |
1 ml |
| Dipotassium hydrogen phosphate |
2.0 |
| Sodium acetate 3H2O |
5.0 |
| Triammonium citrate |
2.0 |
| Magnesium sulphate 7H2O |
0.2 |
| Manganese sulphate 4H2O |
0.05 |
| pH 6.2 ± 0.2 @ 25°C |
|
Description
MRS Broth may be used for tests in the identification of lactobacilli, such as temperature dependence, growth in 4% NaCl, growth in 0.4% Teepol, etc. as recommended by Sharpe, Fryer and Smith1.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
