MRS BROTH (DE MAN, ROGOSA, SHARPE)
A non-selective medium for profuse growth of `lactic acid bacteria’.
|Dipotassium hydrogen phosphate||
|Sodium acetate 3H2O||
|Magnesium sulphate 7H2O||
|Manganese sulphate 4H2O||
|pH 6.2 ± 0.2 @ 25°C||
MRS Broth may be used for tests in the identification of lactobacilli, such as temperature dependence, growth in 4% NaCl, growth in 0.4% Teepol, etc. as recommended by Sharpe, Fryer and Smith1.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.