NUTRIENT AGAR OXOID

NUTRIENT AGAR OXOID

NUTRIENT AGAR

CM0003 (Powder)

 

Suspend 28g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

NUTRIENT AGAR

Code: CM0003 (Powder)

A general purpose medium which may be enriched with up to 10% blood or other biological fluid.

Typical Formula *
gm/litre
`Lab-Lemco’ powder

1.0

Yeast extract

2.0

Peptone

5.0

Sodium chloride

5.0

Agar

15.0

pH 7.4 ± 0.2 @ 25°C

 

Description
Nutrient Agar is a basic culture medium used to subculture organisms for maintenance purposes or to check the purity of subcultures from isolation plates prior to biochemical or serological tests.

In semi-solid form, agar slopes or agar butts, the medium is used to maintain control organisms1.

Nutrient Agar is suitable for teaching and demonstration purposes. It contains a concentration of 1.5% agar to permit the addition of up to 10% blood or other biological fluid, as required. The medium, without additions, may be used for the cultivation of organisms which are not exacting in their nutritional requirements.