NUTRIENT AGAR
Code: CM0003 (Powder)
A general purpose medium which may be enriched with up to 10% blood or other biological fluid.
Typical Formula * |
gm/litre
|
`Lab-Lemco’ powder |
1.0 |
Yeast extract |
2.0 |
Peptone |
5.0 |
Sodium chloride |
5.0 |
Agar |
15.0 |
pH 7.4 ± 0.2 @ 25°C |
Description
Nutrient Agar is a basic culture medium used to subculture organisms for maintenance purposes or to check the purity of subcultures from isolation plates prior to biochemical or serological tests.
In semi-solid form, agar slopes or agar butts, the medium is used to maintain control organisms1.
Nutrient Agar is suitable for teaching and demonstration purposes. It contains a concentration of 1.5% agar to permit the addition of up to 10% blood or other biological fluid, as required. The medium, without additions, may be used for the cultivation of organisms which are not exacting in their nutritional requirements.